Microbiology: Codon During Protein Production
ANSWER ANY THREE QUESTIONS
1. Using the following list of codons describe, using diagrams etc., how information stored in the DNA is translated into a peptide. Be sure to discuss all steps. In other words, use a diagram and give me sequences, transcription and translation steps. Show the sequences of the sense and the other DNA strand, the mRNA and the tRNA’s.
UUU -phenylalanine UCU -serine AUG –initiation/methionine
CUU -leucine ACU -threonine
GUU -valine UAA -Termination
2. a. What is a mutation in molecular terms?
b. If a mutation deletes a base in the genomic DNA discuss how that will affect the reading frame and expression product production.
3. a. Discuss, using the lactose operon, how induction controls enzyme synthesis.
b. Discuss, using the tryptophane operon and how the levels of a key metabolite (tryptophane) controls gene function.
4. a. If you knew the amino acid sequence of a peptide could you use that information to find the gene in a pool of genomic DNA. If so, how might you do this.
b. How does a cell recognize which strand is the sense strand? How does the RNA polymerase enzyme actually know where to start making a message? How about stopping?
5. a. Discuss three ways that bacteria can transfer DNA between cells horizontally.
b. What is the difference between lytic and lysogenic viral replication?
6. Define/identify: codon, anti-codon, transcription, translation, helicase, restriction enzyme topoisomerase, PCR, RNA interference.
You are to answer all questions fully. Any reference material you used should be noted. That would be the textbook chapter and page(s), your notes, any URLs from sites you browsed on the internet.
I would say that most of the reference should from the textbook and notes. All the reference should be solid.
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