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Topic:

The Enterobacteriaceae (Enteric Bacilli)

Essay Instructions:

Week 7 – Review Sheet

Exercise 3 - The Enterobacteriaceae (Enteric Bacilli)

1. What does the term IMViC mean?

2. Why is the IMViC useful in identifying Enterobacteriaceae? Are further biochemical tests necessary

for complete identification?

3. What diagnostic test differentiates Proteus and Providencia species from other Enterobacteriaceae?

4. How is E. coli distinguished from P. vulgaris on MacConkey agar? On a TSI slant?

5. Why is it important to differentiate glucose nonfermenters from Enterobacteriaceae?

Essay Sample Content Preview:

Identifying Enteric Bacilli.
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Identifying Enteric Bacilli.
1 What does the term IMViC mean?
The IMViC acronym is commonly used to identify four distinct biochemical tests that are employed to differentiate various species within the Enterobacteriaceae family. These tests include Indole, Methyl Red, Voges-Proskauer, and Citrate. These tests focus on distinct metabolic pathways of the bacteria. For instance, the Indole test gauges the bacteria's ability to decompose tryptophan, while the Methyl Red test evaluates the bacteria's capacity to carry out mixed acid fermentation. The Voges-Proskauer test checks for acetoin production from glucose, whereas the Citrate test examines the bacteria's ability to use citrate as the primary carbon source.
2 Why is the IMViC useful in identifying Enterobacteriaceae? Are further biochemical tests necessary for complete identification?
The IMViC tests are valuable for distinguishing between different species within the Enterobacteriaceae family of bacteria. These tests offer a quick and dependable method for identifying these bacteria by analyzing their metabolic functions. By detecting the presence or absence of specific enzymes or metabolic pathways unique to particular species, the tests can assist in identifying Enterobacteriaceae. However, due to the possibility of similar results in the IMViC tests between some species, additional biochemical tests such as hydrogen sulfide production or urease activity may be required to achieve complete identification (Kaushik et al.,2018).
3 What diagnostic test differentiates Proteus and Providencia species from other Enterobacteriaceae?
The urease t...
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